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平滑筋細胞系譜トレース マウスの高度な動脈硬化病変における細胞構成の定量的解析
JoVE Journal
Medicine
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JoVE Journal Medicine
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
DOI:

09:06 min

February 20, 2019

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Chapters

  • 00:04Title
  • 00:36Brachiocephalic Artery (BCA) Harvest Preparation
  • 01:56BCA Exposure and Harvest
  • 03:05Immunofluorescent Staining
  • 05:04Confocal Microscopy and Single Cell Counting
  • 07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
  • 08:33Conclusion

Summary

Automatic Translation

動物解剖、組織を埋め込む、断面、染色、腕頭動脈の分析の体系的な方法を含む後期マウスの動脈硬化病変の細胞構成を特徴づけるための標準化されたプロトコルを提案します。atheroprone 平滑筋細胞系譜トレース マウス。

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