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Chapters
- 00:04Title
- 00:36Brachiocephalic Artery (BCA) Harvest Preparation
- 01:56BCA Exposure and Harvest
- 03:05Immunofluorescent Staining
- 05:04Confocal Microscopy and Single Cell Counting
- 07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
- 08:33Conclusion
Biz hayvan diseksiyon, doku gömme, kesit, Boyama, ve brachiocephalic arterler analizini sistematik yöntemleri de dahil olmak üzere son aşama fare aterosklerotik lezyonları hücresel bileşimi karakterize etmek için standart bir protokol öneriyoruz atheroprone düz kas hücre soy izleme fare.
Tags
Quantitative AnalysisCellular CompositionAdvanced Atherosclerotic LesionsSmooth Muscle Cell Lineage-tracingImmunofluorescent StainingPhenotypic CharacterizationQualitative And Quantitative AnalysesCell TypesBrachiocephalic Artery HarvestGravity Perfusion SystemParaformaldehyde FixationCarotid ArterySubclavian ArteryAortic Arch
