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Chapters
- 00:04Title
- 00:36Brachiocephalic Artery (BCA) Harvest Preparation
- 01:56BCA Exposure and Harvest
- 03:05Immunofluorescent Staining
- 05:04Confocal Microscopy and Single Cell Counting
- 07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
- 08:33Conclusion
Wij stellen voor een gestandaardiseerde protocol karakteriseren de cellulaire samenstelling van laat-stadium lymfkliertest atherosclerotische laesies met inbegrip van systematische methoden voor dierlijke dissectie, weefsel insluiten, afdelen, kleuring en analyse van brachiocephalic slagaders van atheroprone gladde spieren cel lineage tracering muizen.
Tags
Quantitative AnalysisCellular CompositionAdvanced Atherosclerotic LesionsSmooth Muscle Cell Lineage-tracingImmunofluorescent StainingPhenotypic CharacterizationQualitative And Quantitative AnalysesCell TypesBrachiocephalic Artery HarvestGravity Perfusion SystemParaformaldehyde FixationCarotid ArterySubclavian ArteryAortic Arch
