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Kvantitativ analyse af cellulære sammensætning i avancerede aterosklerotiske læsioner af glat muskel celle afstamning sporingen mus
JoVE Journal
Medicine
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JoVE Journal Medicine
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
DOI:

09:06 min

February 20, 2019

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Chapters

  • 00:04Title
  • 00:36Brachiocephalic Artery (BCA) Harvest Preparation
  • 01:56BCA Exposure and Harvest
  • 03:05Immunofluorescent Staining
  • 05:04Confocal Microscopy and Single Cell Counting
  • 07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
  • 08:33Conclusion

Summary

Automatic Translation

Vi foreslår en standardiseret protokol for at karakterisere den cellulære sammensætning af sene murine aterosklerotiske læsioner herunder systematiske metoder til animalsk dissektion, væv indlejring, skæring, farvning og analyse af brachiocephalic arterier fra atheroprone glat muskel celle afstamning sporingen mus.

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