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Chapters
- 00:04Title
- 00:36Brachiocephalic Artery (BCA) Harvest Preparation
- 01:56BCA Exposure and Harvest
- 03:05Immunofluorescent Staining
- 05:04Confocal Microscopy and Single Cell Counting
- 07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
- 08:33Conclusion
Vi foreslår en standardiseret protokol for at karakterisere den cellulære sammensætning af sene murine aterosklerotiske læsioner herunder systematiske metoder til animalsk dissektion, væv indlejring, skæring, farvning og analyse af brachiocephalic arterier fra atheroprone glat muskel celle afstamning sporingen mus.
Tags
Quantitative AnalysisCellular CompositionAdvanced Atherosclerotic LesionsSmooth Muscle Cell Lineage-tracingImmunofluorescent StainingPhenotypic CharacterizationQualitative And Quantitative AnalysesCell TypesBrachiocephalic Artery HarvestGravity Perfusion SystemParaformaldehyde FixationCarotid ArterySubclavian ArteryAortic Arch
