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Chapters
- 00:04Title
- 00:36Brachiocephalic Artery (BCA) Harvest Preparation
- 01:56BCA Exposure and Harvest
- 03:05Immunofluorescent Staining
- 05:04Confocal Microscopy and Single Cell Counting
- 07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
- 08:33Conclusion
Vi foreslår en standardisert protokoll betegner mobilnettet sammensetningen av sent murine aterosklerotisk lesjoner inkluderer systematisk dyr disseksjon, vev innebygging, skjæring, flekker og analyse av truncus arterier fra atheroprone glatt muskel celle avstamning sporing mus.
Tags
Quantitative AnalysisCellular CompositionAdvanced Atherosclerotic LesionsSmooth Muscle Cell Lineage-tracingImmunofluorescent StainingPhenotypic CharacterizationQualitative And Quantitative AnalysesCell TypesBrachiocephalic Artery HarvestGravity Perfusion SystemParaformaldehyde FixationCarotid ArterySubclavian ArteryAortic Arch
