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Medicine
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平滑肌细胞近系追踪小鼠晚期动脉粥样硬化病变细胞成分的定量分析
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JoVE Journal
Medicine
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
Please note that all translations are automatically generated.
Click here for the English version.
平滑肌细胞近系追踪小鼠晚期动脉粥样硬化病变细胞成分的定量分析
DOI:
10.3791/59139-v
•
09:06 min
•
February 20, 2019
•
Sidney Mahan
,
Mingjun Liu
,
Richard A. Baylis
3
,
Delphine Gomez
4
1
Heart, Lung, Blood and Vascular Medicine Institute
,
University of Pittsburgh
,
2
Robert M. Berne Cardiovascular Research Center
,
University of Virginia
,
3
Department of Biochemistry and Molecular Genetics
,
University of Virginia
,
4
Division of Cardiology
,
University of Pittsburgh School of Medicine
Chapters
00:04
Title
00:36
Brachiocephalic Artery (BCA) Harvest Preparation
01:56
BCA Exposure and Harvest
03:05
Immunofluorescent Staining
05:04
Confocal Microscopy and Single Cell Counting
07:00
Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
08:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个标准化的方案来表征晚期小鼠动脉粥样硬化病变的细胞组成, 包括动物解剖、组织包埋、切片、染色和腕头动脉分析的系统方法从动脉粥样硬化平滑肌细胞谱系追踪小鼠。
Tags
Quantitative Analysis
Cellular Composition
Advanced Atherosclerotic Lesions
Smooth Muscle Cell Lineage-tracing
Immunofluorescent Staining
Phenotypic Characterization
Qualitative And Quantitative Analyses
Cell Types
Brachiocephalic Artery Harvest
Gravity Perfusion System
Paraformaldehyde Fixation
Carotid Artery
Subclavian Artery
Aortic Arch
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