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Forudsigelse i Vivo nyttelast levering ved hjælp af en blod-hjerne Tumor barriere i et fad
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Predicting In Vivo Payloads Delivery using a Blood-brain Tumor-barrier in a Dish
DOI:

13:34 min

April 16, 2019

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Chapters

  • 00:04Title
  • 02:12Preparation of Astrocytes
  • 04:08Preparation of Endothelial Cells
  • 06:10Preparation of Glioma Cells
  • 07:43Measurement of the BBTB Mimic Permeability (Optional)
  • 08:48BBTB Fluorescence Staining to Detect Nanoparticle Transcytosis
  • 10:08Results: Analysis of In Vivo Payload Delivery using the Blood-brain Tumor-barrier
  • 12:25Conclusion

Summary

Automatic Translation

Drug målretning til centralnervesystemet tumorer er en stor udfordring. Her beskriver vi en protokol for at producere en in vitro-efterligner af blod-hjerne tumor-barrieren ved hjælp af murine og/eller menneskelige celler og diskutere deres relevans for forudsigelighed af centralnervesystemet tumor målretning in vivo.

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