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Journal / Biochemistry / Perfilado e identificación de nitropéptidos…
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Biochemistry

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JoVE Journal Biochemistry
Perfilado e identificación de nitropéptidos ilustrados por Angiotensin A II
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Perfilado e identificación de nitropéptidos ilustrados por Angiotensin A II

Article DOI: 10.3791/59391-v • 07:31 min • June 16th, 2019
June 16th, 2019 •
1,2, 3, 2,4,5,6
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

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El perfilado proteómico de las proteínas nitratodeadas por tirosina ha sido una técnica desafiante debido a la baja abundancia de la modificación de 3-nitrotirosina. Aquí describimos un enfoque novedoso para el enriquecimiento y perfilado de nitropéptidos mediante el uso de Angiotensin II como modelo. Este método se puede extender para otros sistemas in vitro o in vivo.

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Bioquímica Número 148 Nitropéptido Angiotensina II nitrotirosina etiquetado de dimetil ditionato de sodio etiquetado de biotina enriquecimiento
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