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Developmental Biology
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CRISPR/Cas9在iPSC衍生肌肉预祖中恢复肌营养不良素表达的技术
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JoVE Journal
Developmental Biology
CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors
Please note that all translations are automatically generated.
Click here for the English version.
CRISPR/Cas9在iPSC衍生肌肉预祖中恢复肌营养不良素表达的技术
DOI:
10.3791/59432-v
•
07:44 min
•
September 14, 2019
•
Yue Jin
,
Yan Shen
,
Xuan Su
,
Neal Weintraub
,
Yaoliang Tang
1
Medical College of Georgia
,
Augusta University
Chapters
00:04
Title
01:45
Selecting and Harvesting ES-like Cells
03:02
Deletion of Exon 23 in Mouse iPSCs with Two Guide RNAs (gRNAs) coupled with Cas9
05:25
Identification of iPSC Colonies with Exon23 Deletion
06:33
Results: CRISPR/Cas9-mediated Exon23 Deletion
06:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了基于Cas9的外显23删除协议,从Dmd
mdx
小鼠衍生的皮肤成纤维细胞恢复iPSC中的营养不良素表达,并使用Tet-on MyoD激活系统将iPSC直接分化为肌源性祖细胞(MPC)。
Tags
CRISPR/Cas9
Dystrophin
Duchenne Muscular Dystrophy
Induced Pluripotent Stem Cells
Muscle Progenitor Cells
Gene Editing
Stem Cell Therapy
Autologous Cells
Congenital Diseases
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