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一种新型锥形海绵尖电极在幼虫斑马鱼中的电录记录
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Developmental Biology
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JoVE Journal Developmental Biology
Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode
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一种新型锥形海绵尖电极在幼虫斑马鱼中的电录记录

DOI:
10.3791/59487-v

•

07:10 min

•

March 27, 2019

•

, , ,
1School of Biosciences,University of Melbourne, 2Melbourne School of Psychological Sciences,University of Melbourne, 3Department of Optometry and Vision Sciences,University of Melbourne

Chapters

  • 00:04Title
  • 00:57Electrode Preparation
  • 02:44Zebrafish and Sponge Platform Preparation
  • 03:48Animal and Electrode Positioning
  • 05:38Results: Representative Electroretinogram (ERG) A- and B-Wave Amplitude Tracings
  • 06:37Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个协议, 简化测量光诱发视网膜图像反应从幼虫斑马鱼。一种新型锥形海绵尖电极可以使利用电视图 erg 更容易地实现幼虫斑马鱼的视觉发育, 结果可靠, 成本更低。

Tags

ElectroretinogramERGLarval ZebrafishCone-shaped Sponge-tip ElectrodeVisual DevelopmentGenetic Disease ModelsElectrode ManufactureDark AdaptationRinger’s BufferPhotovoltaic Artifacts

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