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Developmental Biology
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一种新型锥形海绵尖电极在幼虫斑马鱼中的电录记录
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Electroretinogram Recording in Larval Zebrafish using A Novel Cone-Shaped Sponge-tip Electrode
Please note that all translations are automatically generated.
Click here for the English version.
一种新型锥形海绵尖电极在幼虫斑马鱼中的电录记录
DOI:
10.3791/59487-v
•
07:10 min
•
March 27, 2019
•
Jiaheng Xie
,
Patricia R. Jusuf
,
Patrick T. Goodbourn
,
Bang V. Bui
1
School of Biosciences
,
University of Melbourne
,
2
Melbourne School of Psychological Sciences
,
University of Melbourne
,
3
Department of Optometry and Vision Sciences
,
University of Melbourne
Chapters
00:04
Title
00:57
Electrode Preparation
02:44
Zebrafish and Sponge Platform Preparation
03:48
Animal and Electrode Positioning
05:38
Results: Representative Electroretinogram (ERG) A- and B-Wave Amplitude Tracings
06:37
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 简化测量光诱发视网膜图像反应从幼虫斑马鱼。一种新型锥形海绵尖电极可以使利用电视图 erg 更容易地实现幼虫斑马鱼的视觉发育, 结果可靠, 成本更低。
Tags
Electroretinogram
ERG
Larval Zebrafish
Cone-shaped Sponge-tip Electrode
Visual Development
Genetic Disease Models
Electrode Manufacture
Dark Adaptation
Ringer’s Buffer
Photovoltaic Artifacts
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