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Påvisning og overvågning af tumor associeret cirkulerende DNA i patientens Biofluider
This content is Free Access.
1Center for Genetic Medicine,Children’s National Health System, 2Institute for Biomedical Sciences,The George Washington University School of Medicine and Health Sciences, 3Dana-Farber Cancer Institute, 4Department of Neurology,University of California San Francisco, 5DIPG Centre of Expertise Zurich,Universitats-Kinderspital Zurich
Chapters
- 00:04Title
- 00:59Target DNA Detection and Quantification
- 03:19Data Analysis
- 05:00Results: Representative Mutation Analyses in Pre-amplified Plasma and Cerebrospinal Fluid (CSF) Cell Free DNA (cfDNA)
- 06:32Conclusion
Her præsenterer vi en protokol til påvisning af tumor somatiske mutationer i cirkulerende DNA til stede i patientens biologiske væsker (biofluider). Vores dråbe digital polymerase kædereaktion (dpcr)-baseret metode muliggør kvantificering af tumor mutation alleliske frekvens (MAF), lette et minimalt invasivt supplement til diagnose og tidsmæssig overvågning af tumorrespons.
