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Journal / Cancer Research / Påvisning og overvågning af tumor associeret…
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Cancer Research

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JoVE Journal Cancer Research
Påvisning og overvågning af tumor associeret cirkulerende DNA i patientens Biofluider
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Påvisning og overvågning af tumor associeret cirkulerende DNA i patientens Biofluider

Article DOI: 10.3791/59721-v • 06:53 min • June 8th, 2019
June 8th, 2019 •
1,2, 1, 1,2, 3, 1, 4,5, 1,2,5
1Center for Genetic Medicine, Children's National Health System, 2Institute for Biomedical Sciences, The George Washington University School of Medicine and Health Sciences, 3Dana-Farber Cancer Institute, 4Department of Neurology, University of California San Francisco, 5DIPG Centre of Expertise Zurich, Universitats-Kinderspital Zurich

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Her præsenterer vi en protokol til påvisning af tumor somatiske mutationer i cirkulerende DNA til stede i patientens biologiske væsker (biofluider). Vores dråbe digital polymerase kædereaktion (dpcr)-baseret metode muliggør kvantificering af tumor mutation alleliske frekvens (MAF), lette et minimalt invasivt supplement til diagnose og tidsmæssig overvågning af tumorrespons.

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Kræftforskning flydende biopsi digital dråbe PCR cirkulerende celle frit DNA plasma serum cerebrospinalvæske
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