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一种新型的尼古丁酰胺二丁二核苷酸校正方法,用于在活细胞中使用Fura-2-模拟进行细胞内Ca
2+
测量
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Biology
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JoVE Journal
Biology
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca
2+
Measurement with Fura-2-Analog in Live Cells
Please note that all translations are automatically generated.
Click here for the English version.
一种新型的尼古丁酰胺二丁二核苷酸校正方法,用于在活细胞中使用Fura-2-模拟进行细胞内Ca
2+
测量
DOI:
10.3791/59881-v
•
05:58 min
•
September 20, 2019
•
Jeong Hoon Lee
,
Jeong Mi Ha
,
Quynh Mai Ho
,
Chae Hun Leem
2,3
1
Department of Physiology
,
University of Ulsan College of Medicine/Asan Medical Center
,
2
Asan Medical Center
,
3
Asan Medical Institute of Convergence Science and Technology
Chapters
00:04
Title
00:41
Mitochondrial Fluoroprobe Loading
01:22
In Situ Isosbestic Fura-2-FF Point Identification
02:40
Background Signal Detection and Cell Area Correction
03:30
R Factor Measurement
04:12
Results: Representative Intracellular Ca
2+
Measurement
05:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
由于NADH和fura-2类似物的激发和发射波长的光谱重叠,在[Ca
2]
的定量测量中,活细胞中两种化学物质的信号干扰是不可避免的。因此,开发了一种新型的NADH信号干扰在线校正方法,以测量[Ca
2]。
Tags
Intracellular Ca2+ Measurement
Fura-2-analog
NADH
Isobestic Point
UV Excitable Probes
Signal Contamination
NADH Correction Method
Live Cell Imaging
Myocytes
Saponin
Calcium-free Solution
Multi-parametric Measurement
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