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Journal / Genetics / Införa en gen knockout direkt i Amastigote skede…
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JoVE Journal Genetics
Införa en gen knockout direkt i Amastigote skede av Trypanosoma cruzi med hjälp av CRISPR/Cas9 systemet
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Införa en gen knockout direkt i Amastigote skede av Trypanosoma cruzi med hjälp av CRISPR/Cas9 systemet

Article DOI: 10.3791/59962-v • 06:13 min • July 31st, 2019
July 31st, 2019 •
1, 1, 1, 1
1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

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Här beskriver vi ett protokoll för att introducera en gen knockout i den extracellulära amastigote av Trypanosoma cruzi, med hjälp av CRISPR/Cas9 systemet. Tillväxten fenotyp kan följas upp antingen genom cellräkning av axenic amastigote kultur eller genom spridning av intracellulära amastigotes efter värd cell invasion.

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Genetik fråga 149 CRISPR/Cas9 Gene knockout Stage-specifika Trypanosoma cruzi Chagas sjukdom kinetoplastid parasit drog mål axenic kultur amastigote
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