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Journal / Biochemistry / 使用多路复用共免疫沉淀对蛋白质相互作用网络动力学进行量化…
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Biochemistry

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JoVE Journal Biochemistry
使用多路复用共免疫沉淀对蛋白质相互作用网络动力学进行量化
Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Journal (Biochemistry)
Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
Journal (Biochemistry)
In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
 
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使用多路复用共免疫沉淀对蛋白质相互作用网络动力学进行量化

Article DOI: 10.3791/60029-v • 07:57 min • August 21st, 2019
August 21st, 2019 •
1,2, 3,4, 5, 6,7,8, 1,2,9
1Center for Integrative Brain Research, Seattle Children's Research Institute, 2Graduate Program in Neuroscience, University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics, University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, 7Department of Surgery, School of Medicine, University of Missouri, 8Department Bioengineering, College of Engineering, University of Missouri, 9Department of Pediatrics, University of Washington

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定量多路复用免疫沉淀 （QMI） 使用流式细胞测定技术，灵敏地检测两个样本之间靶向蛋白-蛋白质相互作用的丰度差异。QMI可以使用少量的生物材料进行，不需要基因工程标签，并可以适应任何先前定义的蛋白质相互作用网络。

Tags

生物化学， 问题 150， IP-FCM， 蛋白质组学， 蛋白质相互作用， 信号， 免疫沉淀， QMI
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