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细菌、酵母和人类细胞中瞬态形成的分子茶龙组件的原位监测
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JoVE Journal Biology
In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
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细菌、酵母和人类细胞中瞬态形成的分子茶龙组件的原位监测

DOI:
10.3791/60172-v

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00:08 min

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September 02, 2019

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, ,
1Department of Biomedical Sciences of Cells & Systems,University of Groningen, 2Department of Anatomy, Faculty of Medicine,University of Colombo, 3Australian Regenerative Medicine Institute (ARMI),Monash University

Chapters

  • 00:04Title
  • 01:00HeLa Cell Preparation
  • 02:34S. cerevisiae Cell Preparation
  • 04:53Proximity Ligation Assay
  • 06:56Results: Transiently Formed Molecular Chaperone Assemblies Captured by PLA in Prokaryotic and Eukaryotic Cells
  • 08:26Conclusion

Summary

Automatic Translation

Automatic Translation

Cognate J-domain 蛋白质与 Hsp70 伴护器合作，协助处理从蛋白质折叠到降解的各种生物过程。在这里，我们描述了一个原位接近结扎测定，它允许监测这些瞬时形成的伴菌机在细菌，酵母和人类细胞。

Tags

Keywords: In Situ MonitoringTransient Protein InteractionsChaperone ComplexesProteostasisProkaryotic And Eukaryotic CellsMicrobial InfectionsProtein AssembliesImmunohistochemistryImmunofluorescenceELISAImmunoprecipitationsPoly-L-LysineParaformaldehydeTriton-X100S. Cerevisiae

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