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Immunology and Infection
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Visualisering af Candida albicans i murine mave-tarmkanalen ved hjælp fluorescerende in situ hybridisering
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization

Visualisering af Candida albicans i murine mave-tarmkanalen ved hjælp fluorescerende in situ hybridisering

DOI:
10.3791/60283-v

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10:08 min

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November 05, 2019

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, ,
1Department of Microbiology and Immunology,University of California, San Francisco, 2Department of Medicine, Division of Infectious Disease,University of California, San Francisco

Chapters

  • 00:04Title
  • 01:28Gastrointestinal Colonization of Mice with C. albicans Using Oral Gavage
  • 03:30Preparation of GI Tissues for Histology
  • 05:50FISH Staining in GI Tract Tissues
  • 08:22Results: FISH-stained C. albicans In Vitro and in Different Gut Compartments
  • 09:17Conclusion

Summary

Automatic Translation

Automatic Translation

Formålet med denne protokol er at visualisere Candida albicans celle form og lokalisering i pattedyrs mave-tarmkanalen.

Tags

Keywords: Candida AlbicansFluorescent In Situ HybridizationGastrointestinal TractMurineVisualizationMicrobiotaInflammatory Bowel DiseaseGavageDissectionAnimal Model

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