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产前Islmn:GFP转基因小鼠的奥库罗运动、特罗赫利尔和脊柱运动神经元的分离与培养
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice
DOI:

10:04 min

November 12, 2019

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Chapters

  • 00:05Title
  • 01:17Ventral Midbrain and Spinal Cord Dissection
  • 06:10Tissue Dissociation
  • 07:23Culture of Purified Primary Motor Neurons
  • 07:59Results: Immunohistochemistry of CN3/CN4 and SMN Isolated Cultures
  • 09:34Conclusion

Summary

Automatic Translation

这项工作提出了一种方案,用于产生原发性耳运动、耳蜗和脊柱运动神经元的同质细胞培养。这些培养物可用于对眼和脊柱运动神经元的形态、细胞、分子和电生理特征进行比较分析。

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