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从
产前Isl
mn:GFP
转基因小鼠的奥库罗运动、特罗赫利尔和脊柱运动神经元的分离与培养
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal
Isl
mn
:GFP
Transgenic Mice
Please note that all translations are automatically generated.
Click here for the English version.
从
产前Isl
mn:GFP
转基因小鼠的奥库罗运动、特罗赫利尔和脊柱运动神经元的分离与培养
DOI:
10.3791/60440-v
•
10:04 min
•
November 12, 2019
•
Ryosuke Fujiki
2,3,4,9
,
Joun Y. Lee
2,10
,
Julie A. Jurgens
2,3,7
,
Mary C. Whitman
5,6
,
Elizabeth C. Engle
2,3,4,5,6,7,8
1
Department of Neurology
,
Boston Children’s Hospital
,
2
FM Kirby Neurobiology Center
,
Boston Children’s Hospital
,
3
Department of Neurology
,
Harvard Medical School
,
4
Medical Genetics Training Program
,
Harvard Medical School
,
5
Department of Ophthalmology
,
Boston Children’s Hospital
,
6
Department of Ophthalmology
,
Harvard Medical School
,
7
Broad Institute of M.I.T. and Harvard
,
8
Howard Hughes Medical Institute
,
9
Department of Neurology
,
Kokura Memorial Hospital
,
10
Department of Genetics
,
Albert Einstein College of Medicine
Chapters
00:05
Title
01:17
Ventral Midbrain and Spinal Cord Dissection
06:10
Tissue Dissociation
07:23
Culture of Purified Primary Motor Neurons
07:59
Results: Immunohistochemistry of CN3/CN4 and SMN Isolated Cultures
09:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项工作提出了一种方案,用于产生原发性耳运动、耳蜗和脊柱运动神经元的同质细胞培养。这些培养物可用于对眼和脊柱运动神经元的形态、细胞、分子和电生理特征进行比较分析。
Tags
Oculomotor Neurons
Trochlear Neurons
Spinal Motor Neurons
Motor Neuron Disease
Amyotrophic Lateral Sclerosis
Islet1 EGF
In Vitro Culture
Embryonic Mice
Motor Neuron Development
Selective Vulnerability
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