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Isolering och kultur av Oculomotor, trochlear, och spinal motoriska nervceller från prenatal ISLmn: GFP transgena möss
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1Department of Neurology,Boston Children’s Hospital, 2FM Kirby Neurobiology Center,Boston Children’s Hospital, 3Department of Neurology,Harvard Medical School, 4Medical Genetics Training Program,Harvard Medical School, 5Department of Ophthalmology,Boston Children’s Hospital, 6Department of Ophthalmology,Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology,Kokura Memorial Hospital, 10Department of Genetics,Albert Einstein College of Medicine
Chapters
- 00:05Title
- 01:17Ventral Midbrain and Spinal Cord Dissection
- 06:10Tissue Dissociation
- 07:23Culture of Purified Primary Motor Neurons
- 07:59Results: Immunohistochemistry of CN3/CN4 and SMN Isolated Cultures
- 09:34Conclusion
Detta arbete presenterar ett protokoll för att ge homogena cellkulturer av primära oculomotor, trochlear, och spinal motoriska nervceller. Dessa kulturer kan användas för jämförande analyser av morfologiska, cellulära, molekylära och elektrofysiologiska egenskaper hos ögon-och spinal motoriska nervceller.
