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Immunology and Infection
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小鼠感染甲型流感病毒期间T卵泡帮助细胞和胚芽中心反应的评价
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
Please note that all translations are automatically generated.
Click here for the English version.
小鼠感染甲型流感病毒期间T卵泡帮助细胞和胚芽中心反应的评价
DOI:
10.3791/60523-v
•
07:07 min
•
June 27, 2020
•
Meng Wang*
1
,
Yuanyuan Huang*
1
,
Wangpeng Gu
3
,
Haikun Wang
1
CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences
,
University of Chinese Academy of Sciences
,
2
School of Life Science
,
University of Science and Technology of China
,
3
State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences
,
University of Chinese Academy of Sciences
Chapters
00:04
Introduction
00:50
Influenza A/Puerto Rico/8/34 H1N1 (PR8) Virus Inoculation
01:52
Mediastinal Lymph Node (mLN) and Splenic Lymphocyte Isolation
03:22
Polyclonal T Follicular Helper (Tfh) Cell Immunostaining
04:58
Results: Representative Tfh Cell and Germinal Center Responses in a Mouse Model of Influenza A Virus Infection
06:27
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文描述了在流感病毒感染小鼠模型中评估Tfh和GC B反应的协议。
Tags
T Follicular Helper Cells
Germinal Center
Influenza A Virus
C57BL/6 Mice
PR8 Virus
Spleen
Mediastinal Lymph Node
Cell Isolation
Biosafety
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