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In vitro stimulation og visualisering af ekstracellulær fælde frigivelse i differentierede humane monocytter-afledte makrofager
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

In vitro stimulation og visualisering af ekstracellulær fælde frigivelse i differentierede humane monocytter-afledte makrofager

English

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9,313 Views

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08:08 min

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November 01, 2019

DOI:

10.3791/60541-v

DOI:
10.3791/60541-v

•

08:08 min
November 01, 2019

•

14 Views
, , ,
1Heart Research Institute, 2Sydney Medical School,University of Sydney, 3Department of Biomedical Sciences,University of Copenhagen

Summary

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Præsenteret her er en protokol til påvisning af makrofag ekstrellulær fælde (MET) produktion i levende cellekultur ved hjælp af mikroskopi og fluorescens farvning. Denne protokol kan udvides yderligere til at undersøge specifikke MARKEDSført protein markører ved immunofluorescens farvning.

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Zhang, Y., Rayner, B. S., Jensen, M., Hawkins, C. L. In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages. J. Vis. Exp. (153), e60541, doi:10.3791/60541 (2019).

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