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Apoptose induktion og detektion i en primær kultur af Havagurk Tarmceller
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JoVE Journal Biology
Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

Apoptose induktion og detektion i en primær kultur af Havagurk Tarmceller

DOI:
10.3791/60557-v

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07:47 min

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January 21, 2020

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, , , , ,
1National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College,Zhejiang Ocean University

Chapters

  • 00:04Title
  • 00:51Intestinal Cell Preparation
  • 02:25Cell Culture
  • 04:17Apoptosis Induction and Detection in A. japonicus Intestinal Cells
  • 06:03Results: Microscopy and Statistical Analysis
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol giver en nem at håndtere metode til kultur tarmcellerne fra havet agurk Apostichopus japonicus og er kompatibel med en række bredt tilgængelige vævsprøver fra marine organismer, herunder ECHINODERMATA, Mollusca, og krebsdyr.

Tags

Primary Cell CultureSea CucumberIntestinal CellsApoptosisCell Culture ProtocolMarine InvertebrateTrypsinCell StrainerCentrifugationCell Culture MediumAntibioticsIncubator

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