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使用结构DNA改变分析和患者衍生异种移植测试癌症的靶向疗法
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Cancer Research
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JoVE Journal Cancer Research
Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts
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使用结构DNA改变分析和患者衍生异种移植测试癌症的靶向疗法

DOI:
10.3791/60646-v

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10:27 min

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July 25, 2020

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,
1Center for Individualized Medicine,Mayo Clinic, 2Department of Molecular Pharmacology and Experimental Therapeutics,Mayo Clinic

Chapters

  • 00:00Introduction
  • 00:59Selection of Therapeutic Targets
  • 03:45Tumor Engraftment and Maintenance
  • 07:15Testing Responses to Genomically Identified Targets in PDX Models
  • 07:46Representative Results
  • 10:02Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一个方案，以测试基于肿瘤的基因组构成选择的靶向疗法的疗效。该协议描述了结构DNA重组的识别和验证，将患者的肿瘤引入小鼠，并测试对相应药物的反应。

Tags

CancerTargeted TherapyStructural DNA AlterationPatient-derived XenograftsWhole Genome SequencingGenomic AlterationDruggable ChangesIn Vivo TreatmentTreatment EfficacyAlgorithmMicrosequencingGene SymbolsPathway AnalysisFisher’s Exact TestDruggable GenesGene Cards

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