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Journal
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Immunology and Infection
/
原生动物寄生虫感染原发性星细胞和微胶质的伴随分离
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
Please note that all translations are automatically generated.
Click here for the English version.
原生动物寄生虫感染原发性星细胞和微胶质的伴随分离
DOI:
10.3791/60680-v
•
09:34 min
•
March 18, 2020
•
Aline de Oliveira Lima Pacheco*
1
,
Marcelo Pires Amaral*
1
,
Ingrid Sancho de Farias
,
Luiza Zainotti Miguel Fahur Bottino
,
Karina Ramalho Bortoluci
1
Centro de Terapia Celular e Molecular (CTCMol)
,
Universidade Federal de São Paulo (UNIFESP)
,
2
Departamento de Ciências Biológicas, Centro de Terapia Celular e Molecular (CTCMol)
,
Universidade Federal de São Paulo (UNIFESP)
Chapters
00:04
Introduction
01:31
Glial Cells Extraction, Maintenance, and Dissociation
07:28
Results: Astrocytes and Microglia Infection
08:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的总体目标是指导如何从中枢神经系统中提取、维持和分离鼠星细胞和微胶质细胞,然后感染原生动物寄生虫。
Tags
Astrocyte Isolation
Microglia Isolation
Glial Cell Extraction
Central Nervous System
Immunological Context
Alzheimer’s
Parkinson’s
Protozoa Parasite
Virus Infection
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