JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
OaAEP1-媒介酵素合成と単分子力分光法のための重合タンパク質の固定化
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Please note that all translations are automatically generated.
Click here for the English version.
OaAEP1-媒介酵素合成と単分子力分光法のための重合タンパク質の固定化
DOI:
10.3791/60774-v
•
08:34 min
•
February 05, 2020
•
Yibing Deng
,
Bin Zheng
,
Yutong Liu
,
Shengchao Shi
,
Jingyuan Nie
,
Tao Wu
,
Peng Zheng
1
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering
,
Nanjing University
Chapters
00:05
Introduction
00:43
Functionalization of Coverslip and Cantilever Surface
04:31
Stepwise Polyprotein Preparation with Controlled Sequences
05:44
AFM Experiment Measurement and Data Analysis
06:54
Results: AFM-based SMFS Measurements, UV-Vis Absorbance Spectra and SDS-PAGE Gel Results
07:51
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
ここでは、タンパク質ポリマーを制御された配列で形成する酵素によってタンパク質モノマーを結合し、単一分子力分光研究のために表面に固定化するプロトコルを提示する。
Tags
OaAEP1
Enzymatic Synthesis
Polymerized Protein
Single-molecule Force Spectroscopy
Protein Oligomer
Polymerization Degree
Protein-based Material
Cysteine
Potassium Chromate
Sulfuric Acid
APTES
Sulfo-SMCC
GL-ELP-50 Cys-protein
Cantilever Surface
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
迅速なワンステップ酵素合成とトレハロース類似体のすべての水精製
生体分子構造決定のための単分子レベルでの高精度FRET
原子間力顕微鏡を用いた単一タンパク質分子の力分光法
磁気ピンセットで G quadruplexes の単一分子マニピュレーション
3色 1 分子 FRET を使用して蛋白質の相互作用の相関関係を研究するには
力の単一分子分光法のための蛋白質の共有結合固定
細胞間の接触でタンパク質間相互作用の分析計蛍光の変動
プロテオーム的分類の単一分子レベルでの均一性の定量化
単一分子FISHによる転写活性アレスの単一細胞解析
リボソームタンパク質合成の単一分子蛍光エネルギー転移研究
Read Article