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Biochemistry
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OaAEP1介导酶合成和聚合蛋白固定,用于单分子力光谱
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Biochemistry
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Please note that all translations are automatically generated.
Click here for the English version.
OaAEP1介导酶合成和聚合蛋白固定,用于单分子力光谱
DOI:
10.3791/60774-v
•
08:34 min
•
February 05, 2020
•
Yibing Deng
,
Bin Zheng
,
Yutong Liu
,
Shengchao Shi
,
Jingyuan Nie
,
Tao Wu
,
Peng Zheng
1
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering
,
Nanjing University
Chapters
00:05
Introduction
00:43
Functionalization of Coverslip and Cantilever Surface
04:31
Stepwise Polyprotein Preparation with Controlled Sequences
05:44
AFM Experiment Measurement and Data Analysis
06:54
Results: AFM-based SMFS Measurements, UV-Vis Absorbance Spectra and SDS-PAGE Gel Results
07:51
Conclusion
Summary
Automatic Translation
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在这里,我们提出了一个方案,通过酶形成蛋白质聚合物与受控序列结合蛋白质单体,并将其固定在表面进行单分子力光谱研究。
Tags
OaAEP1
Enzymatic Synthesis
Polymerized Protein
Single-molecule Force Spectroscopy
Protein Oligomer
Polymerization Degree
Protein-based Material
Cysteine
Potassium Chromate
Sulfuric Acid
APTES
Sulfo-SMCC
GL-ELP-50 Cys-protein
Cantilever Surface
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