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Bioengineering
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使用无细胞细菌提取物、脂质体和乳液转移制备蛋白质生成合成细胞
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer
Please note that all translations are automatically generated.
Click here for the English version.
使用无细胞细菌提取物、脂质体和乳液转移制备蛋白质生成合成细胞
DOI:
10.3791/60829-v
•
09:37 min
•
April 27, 2020
•
Omer Adir*
1,2
,
Noga Sharf-Pauker*
1,2
,
Gal Chen*
1,3
,
Maya Kaduri
,
Nitzan Krinsky
3
,
Janna Shainsky-Roitman
,
Jeny Shklover
,
Avi Schroeder
1
Laboratory for Targeted Drug Delivery and Personalized Medicine Technologies, Department of Chemical Engineering
,
Technion – Israel Institute of Technology
,
2
The Norman Seiden Multidisciplinary Program for Nanoscience and Nanotechnology
,
Technion – Israel Institute of Technology
,
3
The Interdisciplinary Program for Biotechnology
,
Technion – Israel Institute of Technology
Chapters
00:04
Introduction
00:48
Preparation of S30-T7 Lysate
04:24
Preparation of Lipids-in-Oil Solution
05:08
Preparation of Synthetic Cells
07:24
Results: Production of sfGFP and Renilla Luciferase
08:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了用于自下而上制备RNA和产生合成细胞的蛋白质的方法、材料、设备和步骤。合成细胞的内水腔含有S30细菌裂解,封装在脂质双层层(即稳定脂质体)内,使用油水乳液转移方法。
Tags
AI-based Protein Production
Cell-free Bacterial Extracts
Liposome-based Synthetic Cells
Emulsion Transfer
In-situ Therapeutic Protein Synthesis
Metabolic Disease Treatment
Protein Replacement Therapies
Cancer Therapy
Diabetes Treatment
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