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Live Cell Imaging av Microtubule Cytoskeleton og Mikromekanisk manipulering av Arabidopsis Shoot Apical Meristem
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
DOI:

07:52 min

May 23, 2020

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Chapters

  • 00:04Introduction
  • 00:34Plant Growth
  • 01:18Shoot Apical Meristem (SAM) Dissection
  • 02:14Cultured SAM Transfer and Growth
  • 02:57SAM Imaging
  • 04:57Micromechanical SAM Perturbation and Data Analysis
  • 06:23Results: Representative Cortical Microtubule Anisotropy Quantification and Mechanical Ablation in Arabidopsis SAM
  • 07:15Conclusion

Summary

Automatic Translation

Her beskriver vi en protokoll for levende celleavbildning av det kortikale mikrotubulicycyskeletonen ved skyteapokaell meristem og overvåking av responsen på endringer i fysiske krefter.

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