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Journal
/
Bioengineering
/
用于小鼠角膜和康康菲瓦实时成像的自定义多光子显微镜平台
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
Please note that all translations are automatically generated.
Click here for the English version.
用于小鼠角膜和康康菲瓦实时成像的自定义多光子显微镜平台
DOI:
10.3791/60944-v
•
06:53 min
•
May 17, 2020
•
Yueh-Feng Wu
,
Rai-Teng Ye
,
Ming-Kai Pan
3
,
Sung-Jan Lin*
1,3,4,5
,
Hsin-Yuan Tan*
6,7
1
Department of Biomedical Engineering
,
National Taiwan University
,
2
Institute of Pharmacology, College of Medicine
,
National Taiwan University
,
3
Molecular Imaging Center
,
National Taiwan University
,
4
Department of Dermatology
,
National Taiwan University Hospital, and College of Medicine
,
5
Research Center for Developmental Biology and Regenerative Medicine
,
National Taiwan University
,
6
Department of Ophthalmology
,
Chang Gung Memorial Hospital
,
7
College of Medicine
,
Chang Gung University
Chapters
00:04
Introduction
00:40
Multiphoton Microscopy Setup
01:29
Eye Holder Setup
02:38
Z-Serial Image Acquisition
03:43
Image Processing and 3D Reconstruction
04:36
Results: Representative Mouse Cornea and Conjunctiva Imaging
06:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里展示的是一个多光子显微平台,用于实时小鼠眼表面成像。荧光转基因小鼠能够可视化细胞核、细胞膜、神经纤维和眼表面的毛细血管。从胶原结构派生的非线性二谐波生成信号为频闪结构提供了无标签成像。
Tags
Multiphoton Microscopy
Live Imaging
Mouse Cornea
Mouse Conjunctiva
Dual Fluorescent Transgenic Mouse
EGFP
TdTomato
SHG
Stereotaxic Mouse Holder
Eyelid Retraction
Eye Gel
Z-stack Imaging
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