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En tilpasset multiphoton mikroskopi plattform for live imaging av mus cornea og conjunctiva
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1Department of Biomedical Engineering,National Taiwan University, 2Institute of Pharmacology, College of Medicine,National Taiwan University, 3Molecular Imaging Center,National Taiwan University, 4Department of Dermatology,National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine,National Taiwan University, 6Department of Ophthalmology,Chang Gung Memorial Hospital, 7College of Medicine,Chang Gung University
Chapters
- 00:04Introduction
- 00:40Multiphoton Microscopy Setup
- 01:29Eye Holder Setup
- 02:38Z-Serial Image Acquisition
- 03:43Image Processing and 3D Reconstruction
- 04:36Results: Representative Mouse Cornea and Conjunctiva Imaging
- 06:10Conclusion
Presentert her er en multiphoton mikroskopisk plattform for levende mus okulær overflateavbildning. Fluorescerende transgen mus muliggjør visualisering av cellekjerner, cellemembraner, nervefibre og kapillærer innenfor den okulære overflaten. Ikke-lineære andre harmoniske generasjonssignaler avledet fra kollagenstrukturer gir etikettfri bildebehandling for stromal arkitekturer.
