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阿西尔-PEGyl交换凝胶移位测定,用于脑膜蛋白棕榈酸化定量测定
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
DOI:

08:28 min

March 29, 2020

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Chapters

  • 00:04Introduction
  • 00:40Preparation of Mouse Brain Membranes
  • 02:29Acyl-PEGyl Exchange Gel-Shift (APEGS) Assay
  • 06:54Western Blot Analysis of PEGylated Proteins
  • 07:11Results: Palmitoylation of SynDIG Proteins
  • 07:41Conclusion

Summary

Automatic Translation

棕榈化需要以可逆的方式将16碳棕榈酰化物与靶蛋白的半胱氨酸残留物结合。在这里,我们描述了一种生化方法,即acyl-PEGyl交换凝胶移位(APEGS)测定,以研究小鼠脑水化剂中任何感兴趣的蛋白质的棕榈酸化状态。

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