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测量组织肿瘤组织切片的实时药物反应
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Cancer Research
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JoVE Journal Cancer Research
Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices
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测量组织肿瘤组织切片的实时药物反应

DOI:
10.3791/61036-v

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08:27 min

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May 02, 2020

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, ,
1Division of Human Biology,Fred Hutchinson Cancer Research Center, 2Department of Molecular and Cellular Biology,University of Washington, 3Department of Pharmacology,University of Washington

Chapters

  • 00:04Introduction
  • 01:12Tumor Tissue Slice Preparation
  • 04:13Viability Measurement
  • 05:07Drug Effect Evaluation
  • 05:58Results: Representative Tissue Slice Viability Measurement and Drug Response
  • 07:33Conclusion

Summary

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Automatic Translation

我们介绍了一种用于测量有机肿瘤组织切片中实时药物反应的协议。此处概述的实验策略为在临床或小鼠肿瘤的临床或小鼠肿瘤衍生的组织切片上进行中高通量药物屏幕的平台。

Tags

Real-time Drug ResponseViability AssessmentMedium Throughput ScreeningPre-clinical Drug DevelopmentOncologyCancer BiologyNeuroscienceDevelopmental BiologyVibratomeBiopsy PunchesCyanoacrylate Glue

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