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Biology
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骨骼肌成纤维细胞外泌体的分离和表征
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts
Please note that all translations are automatically generated.
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骨骼肌成纤维细胞外泌体的分离和表征
DOI:
10.3791/61127-v
•
06:27 min
•
May 16, 2020
•
Diantha van de Vlekkert
,
Xiaohui Qiu
,
Ida Annunziata
,
Alessandra d’Azzo
1
Department of Genetics
,
St. Jude Children’s Research Hospital
Chapters
00:05
Introduction
00:45
Primary Fibroblast Isolation and Culture
03:04
Purification of Exosomes Using Differential and Ultra-Centrifugation
04:58
Results: Characterization of Purified Exosomes
05:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案说明了1)从成年小鼠腓肠肌中分离和培养原代成纤维细胞,以及2)使用差异超速离心法结合蔗糖密度梯度,然后进行蛋白质印迹分析的外泌体纯化和表征。
Tags
Exosomes
Skeletal Muscle Fibroblasts
Differential Centrifugation
Isolation
Characterization
Biomarkers
Cell Culture
Low Oxygen Conditions
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