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Detecção Simples de Cília Primária por Imunofluorescência
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Detecção Simples de Cília Primária por Imunofluorescência

Article DOI: 10.3791/61155-v • 08:07 min • May 15th, 2020
May 15th, 2020 •
1, 2, 3, 4, 1, 1
1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, 2Department of Clinical Biochemistry and Diagnostics, University Hospital, 3Department of Oncology, Thomayer Hospital, Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University

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Cílios primários são estruturas extracelulares associadas ao centriole. A detecção primária de cílios por coloração imunofluorescente é um procedimento relativamente simples que resulta em imagens de altíssima qualidade. Neste protocolo, os fibroblastos que expressavam cílios primários foram fixados, imunossuídos e imagedos em um microscópio fluorescente ou confocal.

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Biologia Edição 159 cília primária imunofluorescência fibroblastos tubulina alfa acetilada tubulina gama microscopia cultivo in vitro fome de soro irradiação doxorubicina taxol.
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