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1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove,University of Defence, 2Department of Clinical Biochemistry and Diagnostics,University Hospital, 3Department of Oncology, Thomayer Hospital,Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine,Charles University
Chapters
- 00:04Introduction
- 00:40Cell Culture for Immunocytochemistry Staining
- 03:10Immunofluorescent Staining of Primary Cilia In Vitro
- 06:11Results: Fluorescent Imaging of Primary Cilia in Fibroblasts
- 07:26Conclusion
Primära cilier är extracellulära strukturer som är associerade med centriole. Primära cilier upptäckt av immunofluorescent färgning är en relativt enkel procedur som resulterar i extremt högkvalitativa bilder. I detta protokoll, fibroblaster uttrycker primära cilier var fast, immunostained och avbildas i en fluorescerande eller confocal mikroskop.
