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May 16, 2020
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פרוטוקול זה מתאר מודל זיהום אספרגילוס בזחלים זברה. נבגי אספרגילוס הם microinjected לתוך hindbrain של זחלים, טיפול כימי משמש כדי לגרום לדיכוי המערכת החיסונית. התקדמות הזיהום מנוטרת באמצעות מערך הדמיה יומי כדי לפקח על צמיחה פטרייתית ותגובות חיסוניות, כמו גם ספירה של נבגים חיים על ידי ציפוי יחידה יוצר מושבה.
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Cite this Article
Thrikawala, S., Rosowski, E. E. Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions. J. Vis. Exp. (159), e61165, doi:10.3791/61165 (2020).
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