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Infektion af zebrafisk larver med Aspergillus Sporer til analyse af host-patogen interaktioner
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Chapters
- 00:04Introduction
- 00:57Zebrafish Larva Hindbrain Ventricle Microinjection
- 04:21Establishment of Injected and Viable Spore Numbers
- 05:22Drug Treatment of Larvae and Survival Analysis
- 05:53Zebrafish Wounding and Entrapment Device for Growth and Imaging
- 07:23Results: Infection Outcome of Aspergillus Spores Microinjected into Zebrafish Larvae
- 09:07Conclusion
Denne protokol beskriver en Aspergillus infektion model i zebrafisk larver. Aspergillus sporer er microinjected i baghjernen af larver, og kemisk behandling bruges til at fremkalde immunosuppression. Infektionsprogression overvåges via en daglig billeddannelsesopsætning for at overvåge svampevækst og immunrespons samt optælling af levende sporer ved kolonidannelsesenhed, der plating.
