In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

Nereo Kalebic; Barbara Langen; Jussi Helppi; Hiroshi Kawasaki; Wieland  B. Huttner

doi:10.3791/61171

Journal

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Neuroscience

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In Vivo Inriktning av neurala progenitorceller i Iller Neocortex av In Utero Electroporation

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

In Vivo Inriktning av neurala progenitorceller i Iller Neocortex av In Utero Electroporation

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5,978 Views

•

07:03 min

•

May 06, 2020

DOI:

10.3791/61171-v

DOI:

10.3791/61171-v

•

07:03 min

May 06, 2020

•

5938 Views

Nereo Kalebic^1,2, Barbara Langen^1,3, Jussi Helppi¹, Hiroshi Kawasaki⁴, Wieland B. Huttner¹

¹Max Planck Institute of Molecular Cell Biology and Genetics ²Human Technopole ³Landesdirektion Sachsen ⁴Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University

Summary

Presenteras här är ett protokoll för att utföra genetisk manipulation i embryonala iller hjärnan med hjälp av in utero elektroporation. Denna metod möjliggör inriktning av neurala stamceller i neocortex in vivo.

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In Vivo Inriktning av neurala progenitorceller i Iller Neocortex av In Utero Electroporation

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Summary

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