Journal
/
/
In Vivo Quantification du chiffre d’affaires des protéines dans le vieillissement C. Elegans à l’aide de Photoconvertible Dendra2
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2
DOI:

09:45 min

June 13, 2020

,

Chapters

  • 00:04Introduction
  • 00:50Microscope Slide Preparation
  • 01:38Confocal Microscope Setup
  • 03:17C. elegans Mounting
  • 04:06Green Dendra2 Conversion: Data Acquisition at Time Zero
  • 05:35Red Dendra2 Conversion: Data Acquisition at Second Time Point
  • 06:13Converted Dendra2 Image Analysis
  • 07:17Results: Representative Huntingtin (HTT)-D2 Conversion and Degradation
  • 09:08Conclusion

Summary

Automatic Translation

Présenté ici est un protocole pour surveiller la dégradation de la protéine huntingtine fusionnée au fluorophore photoconvertible Dendra2.

Related Videos

Read Article