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恶性疟原虫 通过人工膜喂养进行配子体培养和蚊子感染
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JoVE Journal Biology
Plasmodium falciparum Gametocyte Culture and Mosquito Infection Through Artificial Membrane Feeding
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恶性疟原虫 通过人工膜喂养进行配子体培养和蚊子感染

DOI:
10.3791/61426-v

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09:23 min

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July 03, 2020

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, , , ,
1Johns Hopkins Malaria Research Institute, W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health,Johns Hopkins University

Chapters

  • 00:05Introduction
  • 00:45P. falciparum Gametocyte Culture
  • 02:23Standard Membrane Feeding Assay (SMFA)
  • 04:57Midgut Dissection and Oocyst Load Quantification
  • 06:01Salivary Gland Dissection and Sporozoite Load Quantification
  • 07:00Results: Representative Gametocyte Culture Development
  • 08:42Conclusion

Summary

Automatic Translation

Automatic Translation

对疟疾寄生虫的蚊子阶段进行详细调查对于设计有效的传播阻断策略至关重要。该协议展示了如何有效地培养传染性配子体，然后将这些配子体喂给蚊子以产生 恶性疟原虫的蚊子阶段。

Tags

Plasmodium FalciparumGametocyte CultureMosquito InfectionArtificial Membrane FeedingMalaria TransmissionGametocytemiaExflagellationAnopheles MosquitoesBlood SmearCentrifugationCell CultureMicroscopy

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