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Plasmodium falciparum Gametocyte Kultur og Mosquito Infektion gennem kunstig membran fodring
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Plasmodium falciparum Gametocyte Culture and Mosquito Infection Through Artificial Membrane Feeding

Plasmodium falciparum Gametocyte Kultur og Mosquito Infektion gennem kunstig membran fodring

DOI:
10.3791/61426-v

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09:23 min

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July 03, 2020

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, , , ,
1Johns Hopkins Malaria Research Institute, W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health,Johns Hopkins University

Chapters

  • 00:05Introduction
  • 00:45P. falciparum Gametocyte Culture
  • 02:23Standard Membrane Feeding Assay (SMFA)
  • 04:57Midgut Dissection and Oocyst Load Quantification
  • 06:01Salivary Gland Dissection and Sporozoite Load Quantification
  • 07:00Results: Representative Gametocyte Culture Development
  • 08:42Conclusion

Summary

Automatic Translation

Automatic Translation

Detaljerede undersøgelser af myg stadier af malaria parasitter er afgørende for at designe effektive transmission blokering strategier. Denne protokol viser, hvordan man effektivt kultur infektiøse gametocytter og derefter fodre disse gametocytter til myg til at generere myg stadier af P. falciparum.

Tags

Plasmodium FalciparumGametocyte CultureMosquito InfectionArtificial Membrane FeedingMalaria TransmissionGametocytemiaExflagellationAnopheles MosquitoesBlood SmearCentrifugationCell CultureMicroscopy

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