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En enkelt celle dissociation tilgang til molekylær analyse af urinblæren i musen efter rygmarvsskade
JoVE Journal
Biology
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JoVE Journal Biology
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury

En enkelt celle dissociation tilgang til molekylær analyse af urinblæren i musen efter rygmarvsskade

DOI:
10.3791/61455-v

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09:50 min

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June 17, 2020

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, , , , , , , ,
1Department of Urology,Boston Children’s Hospital, 2Department of Surgery,Harvard Medical School, 3Division of Hematology/Oncology,Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences,Harvard Medical School, 7Vascular Biology Program,Boston Children’s Hospital

Chapters

  • 00:04Introduction
  • 00:57Mouse Preparation
  • 01:48Spinal Cord Injury (SCI)
  • 03:17Tissue Perfusion and Procurement
  • 04:34Cell Dissociation and Single Cell Suspension Preparation
  • 05:59Cell Staining
  • 06:44Flow Cytometry Analysis
  • 07:43Results: Representative Mouse Bladder Collagen Expression and Immune Cell Population Analyses
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

Målet med denne protokol er at anvende en optimeret væv dissociation protokol til en mus model af rygmarvsskader og validere tilgangen til enkelt celle analyse ved flow cytometry.

Tags

Single Cell DissociationMolecular AnalysisUrinary BladderMouseSpinal Cord InjuryFACS AnalysisLaminectomySpinal Cord TransectionBladder Expression

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