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Biology
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脊髄損傷後のマウスにおける膀胱の分子解析のための単一細胞解離アプローチ
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury
Please note that all translations are automatically generated.
Click here for the English version.
脊髄損傷後のマウスにおける膀胱の分子解析のための単一細胞解離アプローチ
DOI:
10.3791/61455-v
•
09:50 min
•
June 17, 2020
•
Hussein Atta*
1,2
,
Ali Hashemi Gheinani*
1,2
,
Amanda Wacker
,
Yaser Heshmati
4,5
,
Alex Bigger-Allen
6
,
George Lambrinos
2
,
Yao Gao
7
,
Diane R. Bielenberg
7
,
Rosalyn M. Adam
2
1
Department of Urology
,
Boston Children’s Hospital
,
2
Department of Surgery
,
Harvard Medical School
,
3
Division of Hematology/Oncology
,
Harvard Medical School Boston
,
4
Dana-Farber Cancer Institute
,
5
Broad Institute
,
6
Biological Biomedical Sciences Program, Division of Medical Sciences
,
Harvard Medical School
,
7
Vascular Biology Program
,
Boston Children’s Hospital
Chapters
00:04
Introduction
00:57
Mouse Preparation
01:48
Spinal Cord Injury (SCI)
03:17
Tissue Perfusion and Procurement
04:34
Cell Dissociation and Single Cell Suspension Preparation
05:59
Cell Staining
06:44
Flow Cytometry Analysis
07:43
Results: Representative Mouse Bladder Collagen Expression and Immune Cell Population Analyses
08:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルの目的は、脊髄損傷のマウスモデルに最適化された組織解離プロトコルを適用し、フローサイトメトリーによる単一細胞分析のアプローチを検証することです。
Tags
Single Cell Dissociation
Molecular Analysis
Urinary Bladder
Mouse
Spinal Cord Injury
FACS Analysis
Laminectomy
Spinal Cord Transection
Bladder Expression
Article
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