Journal
/
/
使用 CRISPR 介质基础编辑器对 BRCA1 变体进行功能评估
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
DOI:

09:22 min

February 28, 2021

, , , ,

Chapters

  • 00:05Introduction
  • 01:03Design and Construction of BRCA1 Targeting gRNAs
  • 03:17Creation of BRCA1 Variants using CRISPR-mediated Base Editing Tools
  • 04:15Sample Preparation for Illumina Next-Generation Sequencing (NGS)
  • 07:01Results: Functional Study of BRCA1 using CRISPR-Cas9 Systems
  • 08:34Conclusion

Summary

Automatic Translation

BRCA1突变患者患癌症的风险较高,这值得准确评估BRCA1变异的功能。在此,我们描述了使用CRISPR介质细胞素基础编辑器对BRCA1变种进行功能评估的协议,该基编辑器支持活细胞中目标C:G到T:A的转换。

Related Videos

Read Article