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JoVE Journal
Biology

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在革兰氏负性细菌中生成跨波子插入库,用于高通量测序
 
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在革兰氏负性细菌中生成跨波子插入库,用于高通量测序

Article DOI: 10.3791/61612-v 08:19 min July 7th, 2020
July 7th, 2020

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

我们描述了一种在Gram-阴性细菌中生成饱和变性突变库的方法,以及随后制备用于高通量测序的DNA安培库。例如,我们关注ESKAPE病原体 ,Acineto细菌鲍曼尼,但这个协议是适用于广泛的革兰阴性生物。

Tags

生物学, 问题 161, 转波, 高通量测序, 格拉姆阴性, 阿辛托细菌鲍曼尼, 科利丁, ESKAPE
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