An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

Yohei Tomita; Zhuo Shao; Bertan Cakir; Yumi Kotoda; Zhongjie Fu; Lois  E.H. Smith

doi:10.3791/61677

Journal

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Biology

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An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

JoVE Journal
Biology

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JoVE Journal Biology

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

DOI:

10.3791/61677-v

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06:10 min

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August 06, 2020

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Yohei Tomita, Zhuo Shao, Bertan Cakir, Yumi Kotoda, Zhongjie Fu³, Lois E.H. Smith

¹Department of Ophthalmology,Boston Children’s Hospital, Harvard Medical School, ²Department of Clinical and Metabolic Genetics,Hospital for Sick Children, University of Toronto, ³Manton Center for Orphan Disease,Harvard Medical School, Boston Children’s Hospital

Chapters

00:04Introduction
00:47Preparation and Experimental Steps
03:26SWIFT-Choroid Computerized Quantification Method
04:25Results: Free Fatty Acid Receptor (FFAR) 4 Suppression Exacerbate Choroidal Neovascularization
05:38Conclusion

Summary

Automatic Translation

This protocol presents choroid sprouting assay, an ex vivo model of microvascular proliferation. This assay can be used to assess pathways involved in proliferating choroidal micro vessels and assess drug treatments using wild type and genetically modified mouse tissue.