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En Ex Vivo Choroid Sprouting Assay av okulär Microvascular Angiogenesis

Article DOI: 10.3791/61677-v • 06:10 min • August 6th, 2020
August 6th, 2020 •
1, 2, 1, 1, 1,3, 1
1Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, 2Department of Clinical and Metabolic Genetics, Hospital for Sick Children, University of Toronto, 3Manton Center for Orphan Disease, Harvard Medical School, Boston Children's Hospital

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Detta protokoll presenterar koridider groning assay, en ex vivo modell av microvascular spridning. Denna analys kan användas för att bedöma vägar som deltar i proliferering choroidal mikrokärl och bedöma läkemedelsbehandlingar med hjälp av vild typ och genetiskt modifierade musvävnad.

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Biologi koroider groning assay endotelceller retinal pigment epitelium RPE choroidal angiogenesis choroidal neovascularization åldersrelaterad makuladegeneration AMD ex vivo
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