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1Cardiovascular Research Institute for Intractable Disease, College of Medicine,The Catholic University of Korea, 2Division of Cardiology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, 3Division of Cardiology, Department of Internal Medicine, Uijeongbu St. Mary’s Hospital, College of Medicine,The Catholic University of Korea
Chapters
- 00:05Introduction
- 01:49Preparation of MSCs and Injectable Gelatin Hydrogels
- 03:38In Situ MSC-loading and Three-dimensional in Vitro Culture
- 04:29Induction of Myocardial Infarction in Mice
- 05:44Intramyocardial Transplantation of MSC-loading GH Hydrogels
- 06:37Results: Improving Stem Cell Retention and Engraftment with Injectable Hydrogels
- 08:31Conclusion
La terapia basada en células madre ha surgido como una estrategia eficiente para reparar los tejidos cardíacos lesionados después del infarto de miocardio. Proporcionamos una aplicación in vivo óptima para el trasplante de células madre utilizando hidrogeles de gelatina que son capaces de ser enzimáticamente cruzados.
Tags
Mesenchymal Stem CellsMSCsInjectable HydrogelsIntramyocardial TransplantationMyocardial InfarctionMurine ModelCell RetentionCell SurvivalGrowth FactorsGenetic MaterialsDrugsIn Vitro ExperimentIn Vivo ExperimentCell CultureCell SuspensionTrypsinTrypan BlueCell CountingGH Conjugate SolutionHydrogen PeroxideDual Syringe
