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Journal / Biochemistry / Co-translationel indsættelse af membranproteiner…
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Biochemistry

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JoVE Journal Biochemistry
Co-translationel indsættelse af membranproteiner i præformerede nanodiscs
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
Journal (Biochemistry)
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Journal (Biochemistry)
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
 
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Co-translationel indsættelse af membranproteiner i præformerede nanodiscs

Article DOI: 10.3791/61844-v • 08:24 min • November 19th, 2020
November 19th, 2020 •
1, 1, 1, 1
1Institute of Biophysical Chemistry, Goethe University Frankfurt

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Co-translationel indsættelse i præformede nanodiscs gør det muligt at studere cellefrie syntetiserede membranproteiner i definerede lipidmiljøer uden kontakt med vaskemidler. Denne protokol beskriver forberedelsen af væsentlige systemkomponenter og de kritiske parametre til forbedring af ekspressionseffektivitet og prøvekvalitet.

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Biokemi udgave 165 In vitro-ekspression cellefri L-CF vaskemiddelfri nanodiscs membranproteiner G-proteinkoblede receptorer proteorhodopsin membrandesign co-translationel membranindsættelse
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