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JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
막 단백질을 미리 형성된 나노디스크에 공동 번역 삽입
Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
Journal (Biochemistry)
Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
Journal (Biochemistry)
Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
 
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막 단백질을 미리 형성된 나노디스크에 공동 번역 삽입

Article DOI: 10.3791/61844-v
November 19th, 2020 •
1, 1, 1, 1
1Institute of Biophysical Chemistry, Goethe University Frankfurt

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November 19th, 2020

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Click here for the English version.

사전 형성된 나노디스크에 공동 번역하여 삽입하면 세제와의 접촉 없이 정의된 지질 환경에서 무세포 합성된 막 단백질을 연구할 수 있습니다. 이 프로토콜은 필수 시스템 구성 요소의 준비와 발현 효율성 및 샘플 품질을 개선하기 위한 중요한 파라미터를 설명합니다.

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